Fused WPBs translocate out of the plane of the plasma membrane during WPB release of contents. (A) Still from an xyz-projected view of WPB exocytosis. Inset shows the region in which the vertical (yz projection) was made in B. Time is relative to fusion (0 s). The dotted line shows the part of the cell in which a yz section was generated. The boxed region is shown at higher magnification and at a number of time points in B. Bar, 10 µm. (B) Time series of the organelle in the box in A as viewed in a vertical section. The short red arrows (0–55 s; GFP-VWF and Lifeact-Ruby time strips) indicate the position of the center of maximum fluorescence intensity within the WPBs or associated actin filaments at the point of fusion (0 s), and the long white arrows show the center at each new position (5–55 s). The WPB appears to translocate to the right. Actin filaments associated with WPBs appear to remain stationary with respect to the substratum. VWF and Lifeact-Ruby are single image planes. Bar, 1 µm.