EVA analysis in various RNAi treatments. (A–H) The distribution of EB1 velocities >1.0 µm/s in RNAi-treated mei-1(ct46) embryos. The insets are the entire velocity distribution; the boxed areas within the insets are enlarged. The dotted lines are the computationally derived Gaussian population with a mean >1.0 µm/s. n = number of cortical EB1 trajectories. Each histogram and population analysis used pooled data (at least five embryos from at least two independent RNAi trials).