Myo2 is present on mitochondria. (A) Mitochondria were isolated from WT cells, purified by sucrose density centrifugation, and analyzed by postembedding immuno–EM using affinity-purified antibodies against Myo2. Bars, 100 nm. (B) WT mitochondria were either left untreated (WT), extracted with high salt (WT + KCl), or subjected to trypsin digestion (WT + trypsin). Mitochondria were purified from the TetO7-myo2 strain grown in the absence (TetO7-myo2 − Dox) or presence (TetO7-myo2 + Dox) of doxycycline. Mitochondria were analyzed by immuno–EM as in A. Between 106 and 341 mitochondria per sample were analyzed for the presence of gold particles. The number of gold particles per organelle was related to the untreated WT sample that was always analyzed in the same experiment. Results are mean values of two independent labeling experiments (Table S3). Individual data points are represented by circles.
