Δmmr1/myo2(LQ) double mutant cells resemble myo2(LQ) single mutants. (A) 10-fold serial dilutions of cell suspensions were spotted on yeast extract/peptone/dextrose plates and incubated at 30°C for 3 d. (B) Cells expressing mtGFP were grown at the indicated temperature in yeast extract/peptone/dextrose medium to the logarithmic growth phase and analyzed by fluorescence microscopy at ambient temperature. (left) DIC image. (right) GFP fluorescence. Bar, 5 µm. (C) Cells were grown as in B, and small buds devoid of mitochondria were quantified. Quantifications are mean values from three independent experiments (n = 100), and error bars indicate standard deviations.