Organelle specificity of myo2 alleles. (A) Cells were analyzed as in Fig. 2 C. (left) DIC of a representative cell. (middle) Maximum intensity projection of a z stack consisting of 10 focal planes. (right) Merge images. (B) Cells expressing a GFP marker for the ER were analyzed as in A with the exception that fluorescence images represent a single focal plane that is focused on the bud. Arrows indicate bud-localized ERs. (A and B) Quantifications are mean values from three independent experiments (n = 100), and error bars indicate standard deviations. (C) Cells coexpressing mtCherry and ER GFP were analyzed as in A. (right) Merged images of mCherry and GFP fluorescence. Arrows indicate bud-localized ERs. (D) Cells expressing mtGFP were stained with the vacuolar marker CMAC and analyzed as in A. (right) Merged images of GFP and CMAC fluorescence and DIC. (E) Cells coexpressing Myo2-GFP-Fis1 and mtCherry were grown to the late logarithmic growth phase and analyzed as in A. (right) A merged image of GFP and mCherry fluorescence. (C–E) Fluorescence images are maximum intensity projections of z stacks consisting of 10 focal planes. Bars, 5 µm.