Effect during mitosis of spindle microtubules on nuclear envelope formation. 2D time-lapse images from mitotic HeLa cells expressing GFP-Sec61β and mCherry-tubulin were acquired during nuclear envelope assembly. (A) Control (also see Video 3) in the absence of nocodazole. (B) Nocodazole treated (also see Video 4). 33 µM nocodazole was added shortly after the onset of anaphase. Arrowheads indicate nuclear envelope initiation at the pole distal region of the chromosome mass. (C) Taxol treated (also see Video 5). 2 µM taxol was added shortly after the onset of anaphase. Arrowheads indicate a gap on the nuclear envelope obstructed by stabilized spindle microtubules. 0 s corresponds to the onset of nuclear envelope formation. Nuclear envelope (NE; green lines) corresponds to regions containing Sec61β on the contour of the chromosome mass, visible as a dark region because of its exclusion of soluble mCherry-tubulin. Bars, 10 µm.