Figure 2.
Figure 2. Transition during mitosis from ER cisternae to the nuclear envelope. (A and B) 2D time-lapse series from a mitotic HeLa cell expressing mCherry-Sec61β and LBR-GFP (A) or GFP-Rtn4HD (B) were acquired during mitotic nuclear envelope assembly. The tracings correspond to the normalized mean fluorescence intensity determined for Sec61β, LBR, and Rtn4HD in the masked regions representing peripheral ER and nascent nuclear envelope (NE). All data from A and B are representative results of three cells. norm., normalized. Bars, 10 µm.

Transition during mitosis from ER cisternae to the nuclear envelope. (A and B) 2D time-lapse series from a mitotic HeLa cell expressing mCherry-Sec61β and LBR-GFP (A) or GFP-Rtn4HD (B) were acquired during mitotic nuclear envelope assembly. The tracings correspond to the normalized mean fluorescence intensity determined for Sec61β, LBR, and Rtn4HD in the masked regions representing peripheral ER and nascent nuclear envelope (NE). All data from A and B are representative results of three cells. norm., normalized. Bars, 10 µm.

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