Mammalian epithelial cells exhibit conserved septin order. (A) MDCK cells expressing SEPT2–conGFP-1-344, which incorporates into septin fibers. The GFP dipoles are measured to be oriented perpendicular to the fiber axis. The white box identifies the magnified region shown in B. Bar, 10 µm. (B) Magnified region of A including panels with scaled azimuth lines and color-intensity schemes. Bar, 1 µm.