Figure 9.
Figure 9. Primary cilium formation is severely impaired in AK1 or AK1B fragment–expressing cells. (A) Schematic of the procedure used for primary cilium induction. (B) Representative images of control (right), flag-AK1 (middle)–, and flag-AK1B (left)–transfected cells maintained in 0.25% serum for 36 h and triple labeled for polyglutamylated tubulin (pglu-tub), AKAP450, and GMAP210. Double merged images are as indicated. Boxes are enlarged on the bottom. T, transfected cell; NT, nontransfected cells. Bars, 7.5 µm. (C) Bar graphs showing the increase on the percentage of cells with primary cilium at 0, 24, and 36 h after serum deprivation. Dotted lines indicate the percentage of cells containing a cilium before starvation. n = 300 from three independent experiments (*, P < 0.001; Tukey HSD). Error bars indicate standard deviations.

Primary cilium formation is severely impaired in AK1 or AK1B fragment–expressing cells. (A) Schematic of the procedure used for primary cilium induction. (B) Representative images of control (right), flag-AK1 (middle)–, and flag-AK1B (left)–transfected cells maintained in 0.25% serum for 36 h and triple labeled for polyglutamylated tubulin (pglu-tub), AKAP450, and GMAP210. Double merged images are as indicated. Boxes are enlarged on the bottom. T, transfected cell; NT, nontransfected cells. Bars, 7.5 µm. (C) Bar graphs showing the increase on the percentage of cells with primary cilium at 0, 24, and 36 h after serum deprivation. Dotted lines indicate the percentage of cells containing a cilium before starvation. n = 300 from three independent experiments (*, P < 0.001; Tukey HSD). Error bars indicate standard deviations.

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