Cell polarization and migration analysis in AK1- and AK1B-expressing cells. (A) Reorientation of the CTR and the GA after wounding in AK1 (top)- and AK1B (bottom)-transfected cells. The white lines indicate the scratch orientation. Pink or white angles indicate nontransfected or transfected cells, respectively. (B) Graph showing the percentage of control, AK1-, and AK1B- transfected cells with the CTR (circles) or the GA (squares) reoriented toward the wound at each time point. Random orientation is expected to be 25%. n ≥ 80 cells for each condition from two independent experiments. (C) Wound-healing assays in either control (top row), AK1 (middle row)-, and AK1B (bottom row)-transfected cells. Frames from time-lapse phase-contrast videos at the indicated times are shown. 13 selected cells were false colored and numbered to better visualize their movement. Tracks indicating frame by frame movement of cells are overlaid in right images. (D) Quantitative analysis of wound-healing assays by using the WimScratch software (Wimasis). Graph represents the percentage of scratch area at each time point in control, AK1-, and AK1B-expressing cells. Values are from three independent experiments. Error bars indicate standard deviations. Bars, 12.5 µm.