Figure 5. The GA of AK1B-transfected... | Rockefeller University Press

Figure 5.

$Figure 5. The GA of AK1B-transfected cells exhibits diminished GT mobility, whereas it is not perturbed in AK1-transfected cells. (A) FRAP experiments were performed in RPE-1 GT-GFP–expressing cells transfected with mCherry-centrin (top row) or cotransfected with mCherry-centrin and either flag-AK1 (middle row) or flag-AK1B (bottom row). (left) Images of selected cells are shown before bleaching (prebleach) with CTR in red and the GA in green. Video frames of the same cells 0 s after bleaching (middle images) and 200 s after bleaching (right images) are shown. Bleached regions are indicated by white squares. (B) Graph showing the percentage of fluorescence recovery intensity for nontransfected, flag-transfected, flag-AK1–, and flag-AK1B–transfected cells. n = 30 for each condition from five independent experiments. (C) Bar graph shows the percentage of GT-GFP mobile fraction under each condition for the same cells (*, P < 0.001; Holm-Sidak). Error bars indicate standard deviations. Bars, 5 µm.$

The GA of AK1B-transfected cells exhibits diminished GT mobility, whereas it is not perturbed in AK1-transfected cells. (A) FRAP experiments were performed in RPE-1 GT-GFP–expressing cells transfected with mCherry-centrin (top row) or cotransfected with mCherry-centrin and either flag-AK1 (middle row) or flag-AK1B (bottom row). (left) Images of selected cells are shown before bleaching (prebleach) with CTR in red and the GA in green. Video frames of the same cells 0 s after bleaching (middle images) and 200 s after bleaching (right images) are shown. Bleached regions are indicated by white squares. (B) Graph showing the percentage of fluorescence recovery intensity for nontransfected, flag-transfected, flag-AK1–, and flag-AK1B–transfected cells. n = 30 for each condition from five independent experiments. (C) Bar graph shows the percentage of GT-GFP mobile fraction under each condition for the same cells (*, P < 0.001; Holm-Sidak). Error bars indicate standard deviations. Bars, 5 µm.