Figure 1.
Figure 1. Sdc2 regulates dendritic arborization. (A) Knockdown of Sdc-2 in mature neurons reduces dendritic arborization. At 13 DIV, cultured hippocampal neurons were transfected with either an Sdc2 shRNA (Sdc2i) or a vector control (pSuper.neo+GFP, abbreviated as pSuper). Dendritic arbors were analyzed at 17 DIV. Because pSuper co-expresses the GFP–neo fusion and the shRNA, GFP signals were used to outline cell morphology. (B) Sdc2 expression induces dendrite outgrowth in young neurons. Sdc2 and GFP were cotransfected into hippocampal neurons at 2 DIV, and cell morphology was analyzed at 5 DIV. Only GFP signal is shown here. The number of primary dendrites and the total dendritic length of transfected neurons were measured in three independent experiments. Equal numbers of transfected neurons were analyzed. Mean values ± SEM are shown (error bars). **, P < 0.005; ***, P < 0.0005. Bars, 30 µm.

Sdc2 regulates dendritic arborization. (A) Knockdown of Sdc-2 in mature neurons reduces dendritic arborization. At 13 DIV, cultured hippocampal neurons were transfected with either an Sdc2 shRNA (Sdc2i) or a vector control (pSuper.neo+GFP, abbreviated as pSuper). Dendritic arbors were analyzed at 17 DIV. Because pSuper co-expresses the GFP–neo fusion and the shRNA, GFP signals were used to outline cell morphology. (B) Sdc2 expression induces dendrite outgrowth in young neurons. Sdc2 and GFP were cotransfected into hippocampal neurons at 2 DIV, and cell morphology was analyzed at 5 DIV. Only GFP signal is shown here. The number of primary dendrites and the total dendritic length of transfected neurons were measured in three independent experiments. Equal numbers of transfected neurons were analyzed. Mean values ± SEM are shown (error bars). **, P < 0.005; ***, P < 0.0005. Bars, 30 µm.

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