DE-cadherin is required for cell wound repair. (A–A′′) DE-cadherin was detected by antibody staining in embryos expressing a nuclear marker (His2Av-mRFP). (A′) Single confocal slices of the embryos in A. (B–B′′) Time-lapse series of an early embryo expressing sChMCA; cadherin-GFP. (C) Western blot analysis of cadherin protein levels in embryos expressing DE-cadherin-GFP. Relative amounts of cadherin are indicated. (D–D′′) Confocal images of an early embryo expressing sChMCA; cadherin-GFP. Cadherin accumulates at the wound edge and colocalizes with actin (arrows). (E–E′′) Time series images of embryos maternally reduced for cadherin (sGMCA; shg^k03401/+; wimp/+). (E′) Kymograph analysis. (F) Quantification of the wound area over time (wt, n = 15; shg^k03401, n = 10). (G) Quantification of wound expansion (P = 0.0009; all results are given as means ± SEM). (H–H′′) Confocal images of an early embryo expressing cadherin-GFP and GAP43-ChFP. Actin and cadherin localization in control (I and I′; n = 12) and Y27632 treated embryos (J and J′; n = 10; sChMCA, cadherin-GFP). (L) Staining showing myosin II and DE-cadherin localization in the early embryo. Bars: (A, B, D, E, H, I, J, and K) 20 µm; (A′′, B′, D′′, H′′, and K′′′) 10 µm; (E′) 5 µm.