Plasma membrane component involvement in single-cell wound repair. Time series of confocal images of early embryos expressing: sChMCA; GFP-Spider (A–A′′), GFP-Spider (B and B′), PH-PLC-GFP (D and D′), and GAP43-ChFP (E–E′′). (A and A′) Actin (red arrowhead) and plasma membrane (green arrowhead) localization at the wound is indicated. (A′′) Single confocal section of specific time points from A, the position of the Z slice is indicated. Fluorescence intensity at the wound edge in SChMCA; GFP-Spider (A′′′), GFP-Spider (C), PH-PLC-GFP (D′′), and GAP43-ChFP (E′′) embryos at specific times after wounding. (F) Cartoon showing the Z planes. Plasma membrane (black line), actin (blue), and myosin (orange) are depicted. Bars: (A, A′′, B, D, E, and E′′) 20 µm; (A′, B′, D′, E′, and E′′′) 10 µm.