Constitutively active RhoA and the N-terminal region of Hax1 rescue neutrophil adhesion. (A) Adhesion assays of control cells stably expressing GFP and Hax1 shRNA A cells expressing GFP, or an shRNA-resistant GFP-Hax1, GFP–Hax1 1–113, and GFP-Hax1Δ113 were plated on 10 µg/ml fibrinogen in the presence or absence of 200 nM fMLP. (B) Adhesion assay of control and Hax1-deficient cells plated on 10 µg/ml fibrinogen in the presence or absence of 200 nM fMLP and 10 µM colchicine for 30 min. (C) Adhesion assay of control and Hax1-deficient cells stably expressing GFP or GFP-RhoA (Q63L). (B and C) ***, P < 0.01; one-way ANOVA with Tukey posttest. (D) Migration velocities of control and Hax1-deficient cells stably expressing GFP, GFP-RhoA (Q63L), GFP-Hax1, GFP–Hax1 1–113, and GFP-Hax1Δ113 during chemotaxis to fMLP in microfluidic chemotaxis devices. Horizontal lines indicate the mean velocity. (A and D) Means with common letters indicate P > 0.05 by ANOVA with Tukey posttest.