Hax1- and RhoA-deficient PLB-985 cells display increased integrin-mediated adhesion. (A) Adhesion assay of differentiated control and Hax1-deficient cells on fibronectin (Fn), fibrinogen (Fbg), and vitronectin (Vn). (B) Adhesion assay of control and Hax1-deficient cells on 10 µg/ml fibrinogen. For antibody blocking, cells were incubated with β2 or αvβ3 integrin function-blocking antibodies as described in Materials and methods. (C) Lentiviral knockdown of RhoA in PLB-985 cells. (D) Adhesion assay of differentiated control and RhoA-deficient PLB-985 cells on 10 µg/ml fibrinogen. (E) Migration velocities of differentiated control and RhoA-deficient cells during chemotaxis in microfluidic gradient generators. P-values were obtained by ANOVA with Tukey posttest (control shRNA, n = 23 cells and RhoA shRNA 1 and RhoA shRNA 2, n = 22 cells; horizontal lines indicate the mean velocity from three independent experiments). *, P < 0.05. Error bars indicate means ± SEM from three independent experiments.