Several hydrophobic residues in the C-terminal region of TGBp3 are crucial for localization. (A) A yeast cell (CWY2765) expressing Rtn1-GFP and mCherry-TGBp3 was imaged for 3D reconstruction. (B) Localization of the indicated GFP-TGBp3 alanine mutants and quantification. (C) Peripheral view of yeast cells coexpressing Rtn1-mCherry and the indicated GFP-TGBp3 mutants or GFP-TGBp2. Bars, 5 µm.