s-FDAP analysis of LatA-induced increase in G-actin concentration. (A) s-FDAP analysis. MCF-7 cells expressing Dp-actin were treated with NRG 2 min after the start of s-FDAP analysis. At 7 min, cells were treated with 1 µM LatA (red) or DMSO (blue). FDAP values were normalized to the mean value during the first 2-min observation. (B) Quantification of the relative FDAP before and after LatA treatment in NRG-treated cells. Data are means ± SEM of 8 cells (vehicle) and 11 cells (LatA) from at least three independent experiments. *, P < 0.05.