Figure 4.
Figure 4. Effect of local accumulation or dilution of actin on s-FDAP analysis. (A) Images of YFP-actin before and after Jasp treatment. MCF-7 cells expressing YFP-actin were treated with 0.3 µM Jasp for 10 min. Bar, 20 µm. (B and C) Effects of local accumulation or dilution of actin on s-FDAP analysis. Time courses of Dp-actin fluorescence intensities at 0 (blue) and 40 ms (red) after photoactivation during s-FDAP analysis are shown for two distinct cells (Cell-1 and -2) treated with 0.3 µM Jasp (C) or untreated (B). Time courses of the relative FDAP from Cell-1 (green) and Cell-2 (magenta) are shown in the bottom panels.

Effect of local accumulation or dilution of actin on s-FDAP analysis. (A) Images of YFP-actin before and after Jasp treatment. MCF-7 cells expressing YFP-actin were treated with 0.3 µM Jasp for 10 min. Bar, 20 µm. (B and C) Effects of local accumulation or dilution of actin on s-FDAP analysis. Time courses of Dp-actin fluorescence intensities at 0 (blue) and 40 ms (red) after photoactivation during s-FDAP analysis are shown for two distinct cells (Cell-1 and -2) treated with 0.3 µM Jasp (C) or untreated (B). Time courses of the relative FDAP from Cell-1 (green) and Cell-2 (magenta) are shown in the bottom panels.

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