s-FDAP analysis of Jasp-induced changes in G-actin concentration. MCF-7 cells were transfected with Dp-actin. The indicated concentrations of Jasp were added 2 min after the start of s-FDAP analysis. Time courses of relative FDAP (thin lines) and the best-fit curves by model fitting (bold lines) are shown. Data are means ± SEM of 13–21 cells from at least three independent experiments.