Figure 2.
Figure 2. Chromosomal passenger proteins inhibit a kinesin-5–dependent brake. (A) The diagram schematizes two possible pathways for CPCs to affect spindle elongation: (1) CPCs positively regulate motors to generate outward sliding forces, or (2) CPCs inhibit a spindle brake. (B) The diagram shows two possible experimental outcomes for double mutant analysis. In pathway 1, a double CPC motor mutant will result in slow (or slower) spindle elongation. In pathway 2, a double CPC mutant will result in faster spindle elongation. (C and D) The indicated double mutants in pGAL1-SLI15 (C) or pGAL1-CTF13 (D) were analyzed as in Fig. 1, and elongation rates are separately compared for each phase. Error bars represent standard deviations from the mean. *, P < 0.5; **, P < 0.005; ***, P < 0.0005.

Chromosomal passenger proteins inhibit a kinesin-5–dependent brake. (A) The diagram schematizes two possible pathways for CPCs to affect spindle elongation: (1) CPCs positively regulate motors to generate outward sliding forces, or (2) CPCs inhibit a spindle brake. (B) The diagram shows two possible experimental outcomes for double mutant analysis. In pathway 1, a double CPC motor mutant will result in slow (or slower) spindle elongation. In pathway 2, a double CPC mutant will result in faster spindle elongation. (C and D) The indicated double mutants in pGAL1-SLI15 (C) or pGAL1-CTF13 (D) were analyzed as in Fig. 1, and elongation rates are separately compared for each phase. Error bars represent standard deviations from the mean. *, P < 0.5; **, P < 0.005; ***, P < 0.0005.

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