Gαi, LGN, and NuMA distribution in dividing neuroepithelial cells. (a–b′) Gαi-Venus (green) shows a homogenous cortical distribution in metaphase (a–a′) and anaphase (b–b′) cells. The three panels in a and b show single optical sections from basal (top) to sub-apical (bottom) levels, whereas a′ and b′ show a Z-view of the same cell. FOP (red) labels centrosomes. (c–d′) LGN-GFP (green) is excluded from the apical and basal cortex in metaphase (c–c′) and anaphase (d–d′) neuroepithelial cells, and forms a wide belt at the lateral cortex. (e–f′) NuMA (green) is excluded from the apical and basal cortex in metaphase (e–e′) and anaphase (f–f′) neuroepithelial cells, and forms a narrow belt at the lateral cortex. Note that f–f′ shows the same cell as d–d′. (g) Double staining for Gαi-Venus (green) and NuMA (red) show the restricted expression of cNuMA at the lateral cortex, whereas Gαi is homogeneous at the cortex. (h) Double staining for LGN-GFP (green) and NuMA (red) shows restricted expression of both proteins at the lateral cortex. Lightning arrows indicate the electroporated product. Bar, 5 µm