Snx16 is required for synaptic growth mediated by BMP and Wg cascades. (A) Quantification of the mean bouton number for muscle 6/7 NMJs from larvae defective in BMP signaling. (B) Quantification of the mean satellite bouton number for muscle 4 NMJs defective in Wg signaling. (C) Representative confocal images of muscle 4 NMJs from Snx16-GFP;tkv^Q199D larvae and quantification of the mean satellite bouton number. Control UAS data are identical to Fig. 5 E. (D) Localization of Tkv^Q199D and endogenous Tkv to Snx16-positive endosomes. Quantification shows muscle 4, segments A2–A4, α-Tkv–integrated density in GFP-positive pixels relative to total NMJ α-Tkv–integrated density in single confocal sections. Error bars are means ± SEM, and the number of samples averaged in each measurement is indicated at the base of each bar. **, P < 0.01; ***, P < 0.005. Bars: (C) 20 µm; (D) 10 µm.