Localization of SNX16 to endosomes at the Drosophila NMJ. (A) elav^C155;UAS-Snx16;UAS-GFP-Rab5 larvae stained with α-SNX16 antibodies. (B) elav^C155;UAS-Snx16-GFP;UAS-mRFP-Rab4 larvae. (C) elav^C155;UAS-Snx16-GFP larvae stained with α-Rab11 antibodies. (D) elav^C155;UAS-Snx16;UAS-tkv-GFP larvae stained with α-SNX16 antibodies. (E) elav^C155;UAS-Snx16-GFP larvae stained with α-Hrs antibodies (also available in the JCB DataViewer). (F) elav^C155;UAS-Snx16-GFP and UAS-Snx16^3A-GFP larvae stained with α-GFP antibodies and processed for immunoelectron microscopy. Bars: (A–E) 5 µm; (F) 100 nm.