Figure 5.
Figure 5. PDGF-BB–stimulated fast cell migration is BARS dependent. (A−E) siRNA-transfected primary mouse fibroblasts were prepared as in Fig. 3 A. (F) Overlay images in A−E were analyzed by a colocalization assay module in MetaMorph software. The percentages of integrin β3 that colocalized with EEA1 are plotted (n = 10). (G) Rab4A and Rab4B protein expression levels from whole-cell lysates were analyzed by SDS-PAGE and Western blotting. (H) siRNA-transfected cells were seeded with or without antiintegrin-blocking antibodies into Transwell inserts and subjected to the PDGF-BB cell migration assay. The number of cells that had migrated through a 0.8-mm2 Transwell membrane was counted (n = 3). (I) Schematics of growth factor–stimulated fast cell migration. CDRS, circular dorsal ruffles; MPS, macropinosomes; EES, early endosomes; RES, recycling endosomes. Error bars represent means ± SD. ***, P < 0.05. Bars: (overlay) 20 µm; (zoom) 5 µm.

PDGF-BB–stimulated fast cell migration is BARS dependent. (A−E) siRNA-transfected primary mouse fibroblasts were prepared as in Fig. 3 A. (F) Overlay images in A−E were analyzed by a colocalization assay module in MetaMorph software. The percentages of integrin β3 that colocalized with EEA1 are plotted (n = 10). (G) Rab4A and Rab4B protein expression levels from whole-cell lysates were analyzed by SDS-PAGE and Western blotting. (H) siRNA-transfected cells were seeded with or without antiintegrin-blocking antibodies into Transwell inserts and subjected to the PDGF-BB cell migration assay. The number of cells that had migrated through a 0.8-mm2 Transwell membrane was counted (n = 3). (I) Schematics of growth factor–stimulated fast cell migration. CDRS, circular dorsal ruffles; MPS, macropinosomes; EES, early endosomes; RES, recycling endosomes. Error bars represent means ± SD. ***, P < 0.05. Bars: (overlay) 20 µm; (zoom) 5 µm.

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