Colocalization of Nem1p-mCherry and droplets in isolated membranes. Membranes derived from postnuclear supernatant of yeast strains containing chromosomally expressed Nem1p-mCherry (left) or plasmid-expressed 3-phosphoglycerate kinase (PGK) CFP-HDEL (right) were stained with BODIPY. The arrowheads point to three examples of colocalization of Nem1p-mCherry and BODIPY puncta on membranes. Bar, 5 µm.