GLH-1 is necessary but not sufficient for the perinuclear association of PGL granules. (A–D) Single XY plane of comma-stage embryos expressing tagged PGL-1 or GLH-1 constructs, immunostained for nuclear pores (red) and either PGL-1 or GLH-1 (green), as noted. (A) Embryo expressing PGL-1 in the intestine. Ectopic PGL-1 granules in the intestine (arrowhead), endogenous PGL-1 granules in the germ cells (arrow). (B) Embryo expressing 3X(FG)-GLH-1 in the intestine. Cytoplasmic granules (arrowhead), perinuclear granules (arrow). (C) hpl-2(tm1489) embryo expressing GLH-1 in the intestine. Cytoplasmic granules (arrowhead), perinuclear granules (arrow). (D) GLH-1 (left) and 3X(FG)-GLH-1 (middle) coexpressed with PGL-1 in intestinal cells. 3XFG from GLH-1 fused to PGL-1 (right). Ectopic PGL-1 granules in the intestine (arrowhead), endogenous PGL-1 granules in the germ cells (arrow). (E) Wild-type and hpl-2(tm1489) L1s grown at 26°C on control bacteria and glh-1(RNAi) bacteria and immunostained for GLH-1 and PGL-3. Germ cells (arrowheads). (F) Germ lines from wild-type worms grown from L1 to adulthood at 26°C on control bacteria and glh-1(RNAi) bacteria.