PGL-1 nucleates granule formation. (A) GFP-tagged and untagged PGL-1 expressed in the intestine. (B and D) GLH-1::mCherry (B) or GLH-3::mCherry (D) coexpressed with PGL-1::GFP in intestinal cells. (C and E) Young larvae expressing GLH-1::GFP (C) or the FG domain of GLH-1 tagged with GFP (E) in intestinal cells. The larvae In the right panels additionally express untagged PGL-1 in intestinal cells (transgenic PGL-1–expressing worms identified by myo-3::mCherry expression in body muscle).