Figure 4.
Figure 4. X dimer mutations stabilize cadherin clusters in low calcium. (A) Time-lapse images (the first and the last frames from videos acquired at 20-s intervals, total duration of 3 min) of cells expressing EcDendra-Δ748-KL (Δ748) and its X dimer mutants K14E (K14E) and Q101A/N143A (Q101/N143) during calcium depletion (numbers indicate min after placing cells in low-calcium/ATP-depletion media; 0, cells right before the media exchange). Note that in low calcium, the parental mutant rapidly exits the junction, whereas Dendra fluorescence in intracellular vesicles (one of which is indicated by an arrow) is not affected. Bars, 40 µM. (B) Kinetics of the release of EcDendra-Δ748-KL mutants (abbreviated as in Fig. 1) from the junctions in low calcium. Note that inactivation of the X dimer interface significantly delays cadherin release (n = 10). The error bars indicate SD.

X dimer mutations stabilize cadherin clusters in low calcium. (A) Time-lapse images (the first and the last frames from videos acquired at 20-s intervals, total duration of 3 min) of cells expressing EcDendra-Δ748-KL (Δ748) and its X dimer mutants K14E (K14E) and Q101A/N143A (Q101/N143) during calcium depletion (numbers indicate min after placing cells in low-calcium/ATP-depletion media; 0, cells right before the media exchange). Note that in low calcium, the parental mutant rapidly exits the junction, whereas Dendra fluorescence in intracellular vesicles (one of which is indicated by an arrow) is not affected. Bars, 40 µM. (B) Kinetics of the release of EcDendra-Δ748-KL mutants (abbreviated as in Fig. 1) from the junctions in low calcium. Note that inactivation of the X dimer interface significantly delays cadherin release (n = 10). The error bars indicate SD.

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