Figure 3.
Figure 3. The strand swap–incompetent mutants form clusters in cadherin-deficient cells. A431D (A and A’) and CHO (B and B’) cells lacking endogenous cadherin were stably transfected with a plasmid encoding the W2A-EcDendra-Δ748-KL mutant and double stained for Dendra (Dn) and β-catenin (β-cat). The complete absence of β-catenin staining in the Dendra-positive junctions confirms their complete deficiency of endogenous cadherin. Bars, 40 µM.

The strand swap–incompetent mutants form clusters in cadherin-deficient cells. A431D (A and A’) and CHO (B and B’) cells lacking endogenous cadherin were stably transfected with a plasmid encoding the W2A-EcDendra-Δ748-KL mutant and double stained for Dendra (Dn) and β-catenin (β-cat). The complete absence of β-catenin staining in the Dendra-positive junctions confirms their complete deficiency of endogenous cadherin. Bars, 40 µM.

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