CENP-F depletion impairs the NE localization of NudE/EL at the G2/M transition in HeLa cells. HeLa cells transfected with scramble or CENP-F siRNA duplexes were preextracted, fixed, and stained with anti-NudE/EL, anti-RanGAP1, and anti–phospho-H3 antibodies. Bars, 10 µm. See also Fig. S2.