Figure 1.
Figure 1. F5 Lckoff CTLs lack killing ability. (A) Western blot of CTL lysates (7 d after stimulation) for F5 WT (1) and Lckoff (2) CTLs probed with antibodies as shown. (B) Immunofluorescence images displaying flattened z stacks of conjugated F5 WT and Lckoff CTLs with NP68-pulsed EL4 targets labeled for Lck (green, i and v), nuclei (blue, ii and vi), and CD8 (white, iii and vii). (C) Killing assay of WT (diamonds) and Lckoff (squares) F5 CTLs using NP68-pulsed EL4 as targets. Error bars show standard deviation from the means of triplicates. The assay is representative of over three independent experiments. Also see Fig. S1. Bars, 10 µm.

F5 Lckoff CTLs lack killing ability. (A) Western blot of CTL lysates (7 d after stimulation) for F5 WT (1) and Lckoff (2) CTLs probed with antibodies as shown. (B) Immunofluorescence images displaying flattened z stacks of conjugated F5 WT and Lckoff CTLs with NP68-pulsed EL4 targets labeled for Lck (green, i and v), nuclei (blue, ii and vi), and CD8 (white, iii and vii). (C) Killing assay of WT (diamonds) and Lckoff (squares) F5 CTLs using NP68-pulsed EL4 as targets. Error bars show standard deviation from the means of triplicates. The assay is representative of over three independent experiments. Also see Fig. S1. Bars, 10 µm.

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