Effects of back extensions on cluster movement are guidance dependent. Mean instantaneous movement of the border cell cluster toward the oocyte binned by size of front extension as in Fig. 3 but divided into cases with only a front extension or both a front and a back extension. The rare events with side extensions as well were excluded. (A) Early control events; genotype, slboGal4, UAS-10×GFP/+. n = 1,336 (total) and 183 (with back extensions). (B) As in A, but with late events. n = 2,350 (total) and 142 (with back extensions). (C) As in A, but in the D-DN genotype, UAS-DN-EGFR/UAS-DN-PVR;slboGal4, UAS-10×GFP/+. The right-most category in A–C is without front extensions. n = 2,675 (total) and 664 (with back extensions). SEM is indicated. In C, the differences between front only and with back extensions are all significant (P < 0.01).