EGFP-CTD colocalization with ERM and F-actin. (A–D) CLSM of transiently EGFP-CTD–expressing HeLa (A, B, and D) and HCC1937 (C) cells labeled with TRITC-phalloidin (for F-actin) and anti-ezrin–radixin–moesin (ERM) antibody detected with Cy5. Colocalization was observed at the PM (A), leading edge (B), membrane ruffles (C), and PM extensions (D). (D) Higher magnifications of three regions (insets) show EGFP-CTD localization with respect to ERM and F-actin.