Compliance mechanosensing does not regionally regulate MT growth lifetimes in 3D ECMs. (A) Comparison of percentages of the population of MTs whose growth dynamics are categorized in four subpopulations (key and percentage values are shown below) in cell branch (left) and peripheral cell body (right) regions. (B) Comparison of mean MT growth speeds (left) and growth excursion lifetimes (right) of cells cultured on 2D or in 3D sandwich gels of the same stiffness (8.7 kPa). (C) Comparison of percentages of the population of MTs whose growth dynamics were categorized in the four subpopulations described in A in cell branch (left) and peripheral cell body (right) regions (percentage values are shown below). (D) A comparison of mean MT growth speeds (left) and growth excursion lifetimes (right) of cells cultured in 8.7 kPa 3D sandwich gels with (3D 8.7 kPa + Blebb) or without the addition of 20 µM blebbistatin (3D 8.7 kPa) or in more compliant 3D sandwich gels (3D 0.7 kPa). **, branches versus cell body; *, between group comparison, P < 0.001. Error bars indicate standard error of the mean.