Figure 1.
Figure 1. The Bro1 domain binds a MIM1-like motif in Snf7. (A) Schematic diagrams of Bro1 and Snf7, including the amino acid sequence alignment of MIM1-like motifs from yeast Snf7 and human CHMP4a. (B) In vitro pull-downs of purified GST or GST-Bro1 domain incubated with bacterial lysates expressing Snf7-HA or Snf7L231A/L234A-HA. GST and GST-Bro1 domain inputs were loaded at 10% of their total used in each pull-down. IB, immunoblotting. (C) Fluorescence and DIC microscopy of vps4Δ, vps4Δ snf7Δ, and vps4Δ snf7L231/234A cells expressing GFP-Bro1 domain or GFP-Bro1. Arrowheads indicate where GFP does (closed) or does not (open) colocalize with FM4-64–labeled class E compartments. Bars, 2 µm.

The Bro1 domain binds a MIM1-like motif in Snf7. (A) Schematic diagrams of Bro1 and Snf7, including the amino acid sequence alignment of MIM1-like motifs from yeast Snf7 and human CHMP4a. (B) In vitro pull-downs of purified GST or GST-Bro1 domain incubated with bacterial lysates expressing Snf7-HA or Snf7L231A/L234A-HA. GST and GST-Bro1 domain inputs were loaded at 10% of their total used in each pull-down. IB, immunoblotting. (C) Fluorescence and DIC microscopy of vps4Δ, vps4Δ snf7Δ, and vps4Δ snf7L231/234A cells expressing GFP-Bro1 domain or GFP-Bro1. Arrowheads indicate where GFP does (closed) or does not (open) colocalize with FM4-64–labeled class E compartments. Bars, 2 µm.

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