Figure 4.
Figure 4. Immobilized GDNF induces neurite outgrowth in rat embryonic hippocampal neurons. (A) Neurite outgrowth in E17 rat hippocampal neurons on immobilized GDNF. Neurons are stained with tubulin-βIII antibodies. Bar, 20 µM. (B) Neurite outgrowth in hippocampal neurons plated on BSA. Neurons are stained with tubulin-βIII antibodies. (C) Quantification of neurite outgrowth on immobilized GDNF, ΔN-GDNF, PSPN, and BSA. Neurons plated on GDNF were untreated (UNTR) or preincubated with heparinase III (H’aseIII), PI-PLC, or 2 µM SFK inhibitor SU6656. As a control, soluble GDNF was added to neurons grown on BSA. Error bars show SEM from three to five independent experiments (*, P < 0.05). (D) Quantification of neurite outgrowth on immobilized GDNF and BSA from wild-type (syndecan-3+/+) and syndecan-3–deficient (syndecan-3−/−) neurons. Error bars show SEM from three independent experiments (*, P < 0.05 by paired t test).

Immobilized GDNF induces neurite outgrowth in rat embryonic hippocampal neurons. (A) Neurite outgrowth in E17 rat hippocampal neurons on immobilized GDNF. Neurons are stained with tubulin-βIII antibodies. Bar, 20 µM. (B) Neurite outgrowth in hippocampal neurons plated on BSA. Neurons are stained with tubulin-βIII antibodies. (C) Quantification of neurite outgrowth on immobilized GDNF, ΔN-GDNF, PSPN, and BSA. Neurons plated on GDNF were untreated (UNTR) or preincubated with heparinase III (H’aseIII), PI-PLC, or 2 µM SFK inhibitor SU6656. As a control, soluble GDNF was added to neurons grown on BSA. Error bars show SEM from three to five independent experiments (*, P < 0.05). (D) Quantification of neurite outgrowth on immobilized GDNF and BSA from wild-type (syndecan-3+/+) and syndecan-3–deficient (syndecan-3−/−) neurons. Error bars show SEM from three independent experiments (*, P < 0.05 by paired t test).

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