p62 puncta associate with the ER independently of FIP200. (A) MEFs stably expressing GFP-ER (GFP fused to cytochrome b5 residues 95–134) were cultured in starvation medium for 1 h. Cells were fixed, permeabilized, and subjected to immunofluorescence microscopy using anti-GFP and anti-p62 antibodies. (B and C) p62 puncta colocalize with VMP1 in FIP200 KO cells. Wild-type (B) and FIP200 KO MEFs (C) stably expressing VMP1-GFP were cultured in starvation medium for 1 h. Cells were stained with anti-p62 antibodies. Signal color is indicated by color of typeface. Bars: (white) 10 µm; (yellow) 1 µm.