Example gallery from the three different systems. Representative ultrastructures found when targeting HIV particles labeled with MA-EGFP on MDCK cells (A–E), mal3p-RFP spots expressed in S. pombe (F–J), and Rvs167-EGFP colocalizing with Abp1-mCherry in S. cerevisiae (K–O). Inner and outer circles correspond to 50% and 80% prediction accuracy, whereas green and red circles stand for GFP and RFP signals, respectively. Bars, 100 nm.