Figure 4.
Figure 4. Application of correlative method to three different systems. Top panels (A–C) show fluorescence microscopy images of resin sections, merge of GFP and RFP channels. Bottom panels (D–F) are electron tomography slices of boxed areas (see also Videos 1–3). (A) HIV particles labeled with MA-EGFP on MDCK cells expressing H2B-RFP. (D) MA-EGFP–labeled HIV particles can be identified in close proximity to filopodia. (B) RFP-mal3p, GFP-atb2p expressed in S. pombe. (E) RFP-mal3p located at a microtubule end. (C) Rvs167-EGFP, Abp1-mCherry expressed in S. cerevisiae. (F) Spot of Rvs167-EGFP colocalizing with Abp1-mCherry marks an endocytic invagination of the plasma membrane. Inner and outer circles correspond to 50% and 80% prediction accuracy, whereas green and red circles stand for GFP and RFP signals, respectively. Bars: (A–C) 5 µm; (D–F) 100 nm.

Application of correlative method to three different systems. Top panels (A–C) show fluorescence microscopy images of resin sections, merge of GFP and RFP channels. Bottom panels (D–F) are electron tomography slices of boxed areas (see also Videos 1–3). (A) HIV particles labeled with MA-EGFP on MDCK cells expressing H2B-RFP. (D) MA-EGFP–labeled HIV particles can be identified in close proximity to filopodia. (B) RFP-mal3p, GFP-atb2p expressed in S. pombe. (E) RFP-mal3p located at a microtubule end. (C) Rvs167-EGFP, Abp1-mCherry expressed in S. cerevisiae. (F) Spot of Rvs167-EGFP colocalizing with Abp1-mCherry marks an endocytic invagination of the plasma membrane. Inner and outer circles correspond to 50% and 80% prediction accuracy, whereas green and red circles stand for GFP and RFP signals, respectively. Bars: (A–C) 5 µm; (D–F) 100 nm.

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