Ago2 is necessary for MTD-induced inhibition of HIF-1α translation. (A) Ago2 mRNA expression by qRT-PCR after stable expression of Ago2 (shRNA; mean ± SEM; n = 2; ***, P < 0.001) or control shRNA (Neg Ctrl). (B) Immunoblot of HeLa cells transfected as in A and treated with 25 nM TX overnight. (right) A more intense scan of lanes 5 and 6 is depicted as a result of sample underloading. (C) Ago2-GFP is visualized in fixed untreated or TX-treated HeLa–GFP-Ago2 cells transfected with Ago2 siRNA (top) and stained for GE-1 (bottom). Bar, 10 µm.