Figure 7.
Figure 7. Role of HIF-1α–targeting miRNAs in the microtubule-dependent translation of HIF-1α. (A) HIF-1α immunoblot of MCF7 cells transfected with each pre-miR, a combination of all four, or a Cy3-labeled negative control (Cy3 Ctrl) and treated with 10 µM proteasome inhibitor MG-132 for 4 h. (B) HIF-1α mRNA expression quantified by qRT-PCR after transfection of all HIF-targeting miRNAs (miRs) in combination (mean ± SEM; n = 2). (C) Relative expression of Ago2-bound miRNAs quantified by qRT-PCR from HeLa–GFP-Ago2 cells treated overnight with 25 nM TX, glucose starvation (left; mean ± SEM; n = 3), or 1 µM Noc followed by a 6-h drug washout (right; mean ± SEM; n = 2, *, P < 0.05; **, P < 0.01). (D) Endogenous miRNA expression was assessed by qRT-PCR in HeLa cells after overnight treatment with 25 nM TX or 25 µM 2ME2.

Role of HIF-1α–targeting miRNAs in the microtubule-dependent translation of HIF-1α. (A) HIF-1α immunoblot of MCF7 cells transfected with each pre-miR, a combination of all four, or a Cy3-labeled negative control (Cy3 Ctrl) and treated with 10 µM proteasome inhibitor MG-132 for 4 h. (B) HIF-1α mRNA expression quantified by qRT-PCR after transfection of all HIF-targeting miRNAs (miRs) in combination (mean ± SEM; n = 2). (C) Relative expression of Ago2-bound miRNAs quantified by qRT-PCR from HeLa–GFP-Ago2 cells treated overnight with 25 nM TX, glucose starvation (left; mean ± SEM; n = 3), or 1 µM Noc followed by a 6-h drug washout (right; mean ± SEM; n = 2, *, P < 0.05; **, P < 0.01). (D) Endogenous miRNA expression was assessed by qRT-PCR in HeLa cells after overnight treatment with 25 nM TX or 25 µM 2ME2.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal