MTDs induce P-body formation. (A) Live cell imaging of HeLa–GFP-Ago2 cells treated with 100 µM 2ME2, 1 µM TX, or 30 µM Noc for 1 h. (B) HeLa–GFP-Ago2 (green) cells treated with 25 µM 2ME2 or 50 nM TX overnight, fixed, and stained for GE-1 (red) and α-tubulin (white). Arrows show colocalization of Ago2 and GE-1. (C) The number of Ago2 foci per cell was quantified (100 cells/condition) and plotted as percentage of cells with either three or less or more than three P-bodies (mean ± SEM; n = 3; **, P < 0.01). (D) Immunoblot of HeLa–GFP-Ago2 cells treated as in B and followed by 4-h hypoxia. Bars, 10 µm.