HIF-1α mRNA associates with cellular microtubules. (A) HIF-1α MBs incubated at 37°C for 1 h with a complementary (C) or sense (S) DNA ODN or total RNA from MCF7 cells. Relative fluorescence units (RFU) are represented as mean ± SEM. (B) MCF7–GFP-tub (white) cells fixed and processed for MB (red) hybridization. (zoom) Higher magnification views of the boxed area are shown. Two representative untreated cells are shown. (C) MCF7–GFP-tub (white) cells transfected with 100 nM HIF-1α MB (red) overnight, treated with 100 µM 2ME2 or 100 nM TX (1 h) and imaged continuously for 60 s. Arrows show inhibition of HIF-1α movement. (max) Stack arithmetic shows trajectory of MB movement. (max-zoom) Higher magnification view of max from boxed areas in the first column (Videos 1–4). (D) Anti-GFP was used to immunoprecipitate lysates from MCF7–GFP-tub cells treated overnight with 25 µM 2ME2 or 25 nM TX. HIF-1α, p53, and GAPDH mRNA expression are shown as mean fold change ± SEM (n = 3). Bars, 10 µm.