Figure 2. Microtubule disruption is... | Rockefeller University Press

Figure 2.

$Figure 2. Microtubule disruption is required for drug-induced inhibition of HIF-1α translation. (A and B) Parental 1A9 (A) and 1A9/PTX10 (B) cells treated with 10 nM TX overnight and subjected to sucrose gradient fractionation. The percentage of HIF-1α or GAPDH mRNA/fraction is plotted for each cell line and drug treatment. (bottom) HIF-1α and GAPDH protein levels in 1A9 (A) and 1A9/PTX10 (B) cells treated with 10 nM TX overnight assessed by immunoblotting after 4-h hypoxia. (C and D) Similar experiments performed using 1A9 (C) or 1A9/2MRC (D) cells to assess the HIF-1α polysome profile after 10 µM 2ME2 treatment. (bottom) Immunoblot of HIF-1α and GAPDH after 4-h hypoxia (Table I).$

Microtubule disruption is required for drug-induced inhibition of HIF-1α translation. (A and B) Parental 1A9 (A) and 1A9/PTX10 (B) cells treated with 10 nM TX overnight and subjected to sucrose gradient fractionation. The percentage of HIF-1α or GAPDH mRNA/fraction is plotted for each cell line and drug treatment. (bottom) HIF-1α and GAPDH protein levels in 1A9 (A) and 1A9/PTX10 (B) cells treated with 10 nM TX overnight assessed by immunoblotting after 4-h hypoxia. (C and D) Similar experiments performed using 1A9 (C) or 1A9/2MRC (D) cells to assess the HIF-1α polysome profile after 10 µM 2ME2 treatment. (bottom) Immunoblot of HIF-1α and GAPDH after 4-h hypoxia (Table I).