Figure 4.
![Figure 4. The flash reduced release only if applied before or during the depolarizing pulse. [CCh*]local = 4 µM. [TTX]o = 0.5 µM. Experimental protocols beneath EPSCs and corresponding columns. EPSCs without (solid line) and with (dashed line) flash. (A–C) Same site, each trace is an average of five repetitions. Test pulse, −1.0 μA, 0.2 ms. Flash was applied 1 ms before (A), in the middle of (B), and 0.05 ms after (C) the test pulse. (D) Average of 14 experiments as in A–C. From left to right, 100 ± 2%, 54 ± 2%, 83 ± 5%, and 97.5 ± 4% of control (significance indicated above bars).](https://cdn.rupress.org/rup/content_public/journal/jcb/192/1/10.1083_jcb.201007053/6/jcb_201007053_lw_fig4.jpeg?Expires=1771206800&Signature=YRGuXvNudycJInMWVjygi0A~awattHrTAx1mTZ4TpD-blxk1p1vThEAvhgdDCpJuBbalxgNTyXFvYIsld5X6z9VoCHa7crsFuVRTjABu05PJ1wg2Xwx1C7yTKKokj03XRMXGzzGGlFe~nDuV1qKmYHGD6ddnx68sa2y0n0QzuGnQ29e-ZFlIQPQEGBmH2MLd0nMziGdBj~IBYlbdqHOFWtEjLTbZHO3y6DS-ZwJjVfxyXR31yEld9b7ZG7SmE2XZSM88NsN5N0R2kLgKTv2hlhhwAWZ0K2qnPvXT-~4flux9Zc5Qymc7NW1FsiWUbt91t-EZFOdWlfbR-RWw60ShZw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The flash reduced release only if applied before or during the depolarizing pulse. [CCh*]local = 4 µM. [TTX]o = 0.5 µM. Experimental protocols beneath EPSCs and corresponding columns. EPSCs without (solid line) and with (dashed line) flash. (A–C) Same site, each trace is an average of five repetitions. Test pulse, −1.0 μA, 0.2 ms. Flash was applied 1 ms before (A), in the middle of (B), and 0.05 ms after (C) the test pulse. (D) Average of 14 experiments as in A–C. From left to right, 100 ± 2%, 54 ± 2%, 83 ± 5%, and 97.5 ± 4% of control (significance indicated above bars).
