Figure 9.
Figure 9. Spindle assembly factors are misregulated after PPP6C depletion. (A) HeLa cells were transfected for 48 h with control or PPP6C si08 duplexes before fixation. Cells were stained using DAPI to detect DNA and antibodies to α-tubulin (Tub), Eg5, and NuMa. (B) Control and PPP6C-depleted cells treated with solvent (DMSO) or 10 µM MLN8237 for 10 min were fixed and then stained for Eg5, NuMa, tubulin (not depicted), and with DAPI to detect DNA. Bars, 10 µm. In both panels, KIF11/Eg5, NuMa, and tubulin signals were measured for the spindle region in ImageJ. KIF11/Eg5 and NuMa signals were normalized for tubulin and are plotted in the graph (n = 12). Error bars indicate the standard error of the mean. Arrowheads mark fragmented spindle poles.

Spindle assembly factors are misregulated after PPP6C depletion. (A) HeLa cells were transfected for 48 h with control or PPP6C si08 duplexes before fixation. Cells were stained using DAPI to detect DNA and antibodies to α-tubulin (Tub), Eg5, and NuMa. (B) Control and PPP6C-depleted cells treated with solvent (DMSO) or 10 µM MLN8237 for 10 min were fixed and then stained for Eg5, NuMa, tubulin (not depicted), and with DAPI to detect DNA. Bars, 10 µm. In both panels, KIF11/Eg5, NuMa, and tubulin signals were measured for the spindle region in ImageJ. KIF11/Eg5 and NuMa signals were normalized for tubulin and are plotted in the graph (n = 12). Error bars indicate the standard error of the mean. Arrowheads mark fragmented spindle poles.

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