Abnormal chromosome segregation in PPP6C-depleted cells. (A and B) HeLa (A) and MRC-5 (B) cells were transfected with control and PPP6C siRNA duplexes for 24–72 h. The cells were fixed and then stained with DAPI, tubulin (green), and CREST antibodies. Bars, 10 µm. (C) The same samples were also Western blotted to test for PPP6C depletion. Molecular mass is given in kilodaltons. (D and E) Control and PPP6C-depleted HeLa (D) and MRC-5 (E) cells in anaphase and telophase are shown. (F) HeLa cells were transfected with all three SAPS domain siRNA duplexes for 72 h. The cells were fixed and then stained with DAPI, tubulin (green), and CREST antibodies. Cells in anaphase and telophase are shown. (D–F) Bars, 5 µm. In all panels, arrowheads mark lagging chromosomes, and these regions are shown in the inset enlargements.