The formation of ring canals is compromised in nesd male and female germline cells. (A) Testes dissected from either wild-type or nesd¹ homozygous male pupae were fixed and stained to detect Pav-KLP, tubulin (tub), and DNA (blue in the merged image). The arrowhead indicates a remnant of a ring canal in the nesd¹ spermatocyte. (B) Testes dissected from either wild-type or nesd¹ homozygous male pupae were fixed and stained to detect Anillin, tubulin, and DNA. The arrowhead indicates an abnormal CR in the nesd¹ spermatocyte. The ring canals marked by the arrows were probably generated during the four spermatogonial mitoses as judged by their position in the cyst. (C) Stage 10 egg chambers from wild-type and nesd¹ ovaries stained for F-actin and DNA. The arrows mark an aberrant ring canal in a binucleate nurse cell. Magnifications are shown in the insets. (D) Testes expressing a nesd::GFP transgene were fixed and stained to detect GFP, Pav-KLP, and DNA. (A, B, and D) Bars, 10 µm. (E) Stage 9 egg chamber dissected from flies expressing a nesd::GFP transgene and fixed and stained to detect GFP, F-actin, and DNA. (D and E) The arrows indicate ring canals. (C and E) Bars, 50 µm.