nesd mutant spermatocytes show late cytokinesis failure. (A) Selected frames from time-lapse sequences of live spermatocytes expressing an sqh::GFP transgene in either a wild-type (sqh::GFP) or nesd1 (nesd1;sqh::GFP) mutant background. DIC images are shown at the top, and the relative fluorescence images are shown at the bottom. The arrowheads indicate the CR after furrow collapse. Time relative to the end of anaphase (when chromosomes reached the poles) is indicated at the top. (B) Furrow ingression dynamics during cytokinesis in wild-type (sqh::GFP) and nesd1 (nesd1;sqh::GFP) spermatocytes plotted over time. Furrow diameters were measured from eight wild-type and 10 nesd mutants (including the cells shown in Fig. 7 A). The error bars indicate standard deviations. (C) Images of live spermatocytes expressing a PLCδ-PH::GFP transgene in either a wild-type (PLCδ-PH::GFP) or nesd1 (nesd1;PLCδ-PH::GFP) mutant background. DIC images are shown on the right, and relative fluorescence images are shown on the left. The arrowheads indicate the invaginating membranes. Bars, 10 µm.
